Effect of Heparin on the In Vivo Release and Clearance of Human
نویسندگان
چکیده
Intravenous injection of heparin (100 U/kg) into normal volunteers resulted in an increase of platelet factor 4 (PF4) level in platelet-poor plasma from a mean value of 1 8.1 ± 6.6 ng/ml before the injection to 257.9 ± 68.3 ng/ml at 5 mm after injection. PF4 antigen isolated from “postheparin plasma” by adsorption on heparin-agarose and elution with 2.0 M NaCI and “authentic PF4” isolated from human platelets showed identical patterns of migration as determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Material released by washed human platelets was injected intravenously into rats. The clearance of PF4 followed a biphasic exponential pattern. The half-lives (TV2) for the fast and slow components for control rats were 1 .2 and 1 7.1 mm. Heparin significantly
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